WPI PZMIII Bedienerhandbuch

PV820/PV830Pneumatic PicoPumpWorld Precision InstrumentsINSTRUCTION MANUALSerial No._____________________101614www.wpiinc.com

6 World Precision InstrumentsINSTRUMENT DESCRIPTIONSecondary Vent Port(Vacuum/Atmosphere)Seconda(VacuumSSolenoid InputSelector SwitchSoSSSHold Pressu

PV820/PV830 Pneumatic PicoPumpWorld Precision Instruments 7!CAUTION: The regulators leak a small amount of gas as a part of their normal operation. T

8 World Precision Instruments Typical SetupsMost of the time, you will use one of two setups on the PicoPump:Purpose2nd Vent Port Selector Switch*So

PV820/PV830 Pneumatic PicoPumpWorld Precision Instruments 9Range Switch/Period Dial–The duration of the solenoid open time, in the timed mode, is det

10 World Precision InstrumentsInstalling Hard Tubing Quick Connect Fitting Collar1/4 in. TubingTo insert, push the tubing in firmly. To remove, push i

PV820/PV830 Pneumatic PicoPumpWorld Precision Instruments 11Pressure Input–Connect the Pressure Input (located at the back of the instrument) to a s

12 World Precision InstrumentsGasket Color Green Black Red WhitePipette Diameter (mm) 1.0 1.2 1.5 1.653. Connect the other end of the small tubing t

PV820/PV830 Pneumatic PicoPumpWorld Precision Instruments 13NOTE: The pressure of capillary action is determined by the inner diameter of the glass c

14 World Precision InstrumentsThe most important of these steps is cleaning the glass. Small amounts of dust or grease can easily clog micron-sized t

PV820/PV830 Pneumatic PicoPumpWorld Precision Instruments 15For ejected volumes less than 1nL, visual inspection in air proves to be diffi cult due to

Other WPI Favorites M3301 Manual MicromanipulatorThe world’s most widely used micromanipulatorWeighing just 550 grams and employing a slim space-savi

16 World Precision InstrumentsSolenoid Input Selection Switch. When the pipette is full, set the Solenoid Input Selection Switch to Hold.7. Set the D

PV820/PV830 Pneumatic PicoPumpWorld Precision Instruments 17Multibarrel MicroinjectionFor injection with multibarrel micropipette, the PolyFil mult

18 World Precision Instruments ACCESSORIESPart Number Description3260 Foot Switch2932 Rack Mount Kit, 31 2-in. high (PV800 & PV820)2933 Rack M

PV820/PV830 Pneumatic PicoPumpWorld Precision Instruments 19 TROUBLESHOOTING THE HOLD PRESSUREIf the Hold regulator can’t reduce the pressure low en

20 World Precision Instruments SPECIFICATIONSThis unit conforms to the following specifi cations:PV820 PV830PRESSUREPressure Input Pressure OutputPuls

PV820/PV830 Pneumatic PicoPumpWorld Precision Instruments 21 APPENDIX A: FLOW DIAGRAMSThe following diagrams depict how air and vacuum are routed thr

22 World Precision InstrumentsSchematicsPV820 Block DiagramNO NCPressure InputFilterPressure RegulatorEjectPressureGauge Hold Pressure GaugePressure

PV820/PV830 Pneumatic PicoPumpWorld Precision Instruments 23 APPENDIX B: DROPLET VOLUMEUse the chart below to gauge the volume of a droplet and the t

24 World Precision InstrumentsBIBLIOGRAPHYR.E. McCaman, D.G. McKenna and J.K. Ono, A Pressure System for Intracellular and Extracellular Ejections of

PV820/PV830 Pneumatic PicoPumpWorld Precision Instruments 25INDEXSymbols20-turn regulator 13316 35430-ALL 3Aargon 11atmospheric air 7Ccapillary

PV820/PV830 Pneumatic PicoPumpWorld Precision Instruments iiiCopyright © 2014 by World Precision Instruments, Inc. All rights reserved. No part of th

26 World Precision InstrumentsDECLARATION OF CONFORMITY

PV820/PV830 Pneumatic PicoPumpWorld Precision Instruments 27* Electrodes, batteries and other consumable parts are warranted for 30 days only from th

World Precision Instruments, Inc.USAInternational Trade Center, 175 Sarasota Center Blvd., Sarasota FL 34240-9258Tel: 941-371-1003 • Fax: 941-377-5428

iv World Precision Instruments

PV820/PV830 Pneumatic PicoPumpWorld Precision Instruments 1ABOUT THIS MANUALThe following symbols are used in this guide:This symbol indicates a CAUT

2 World Precision Instrumentsamount of time that the injection pressure is applied to the output port. Time intervals can range from 10 seconds down

PV820/PV830 Pneumatic PicoPumpWorld Precision Instruments 3 Parts ListAfter unpacking, verify that there is no visible damage to the instrument. Ver

4 World Precision InstrumentsFig. 5— Each PicoPump is supplied with a 5430-ALL PicoNozzle (Version 2) kit. Legacy PicoNozzle KitsNOTE: The legacy Ver

PV820/PV830 Pneumatic PicoPumpWorld Precision Instruments 5To mount the micropipette, pulled capillary glass may be inserted in the holder. A screw c